Authors: G. Somlyai, A. Kovacs, I. Guller, Z. Gyongyi, etc
EJC Supplements 8, No. 5 (2010), 155-225
It is known that the deuterium/hydrogen (D/H) mass ratio is the largest of stable isotopes of the same element, causing differences in the physical and chemical behavior between the two hydrogen isotopes. Although the concentration of D is more than 10 mM in living organisms the possible role of D had not been investigated for 6 decades after it’s discovery in the early 30’s.
In order to investigate the possible role if naturally occurring D in living organisms, in cell growth and tumour development, D-depleted water (DDW) was used.The experiments with DDW revealed that due to D-depletion the cell growth of various cell lines (PC-3 human prostate; Mda, human breast; HT-29, human colon; M14, human melanoma) were inhibited in vitro.
DDW caused tumour regression in xenotransplanted mice (MDA and MCF-7, human breast; PC-3) and induced apoptosis in vitro and in vivo. Deuterium depletion inhibited the expression of certain genes (c-myc, H-ras, COX-2) having key role in tumour development.Breast tumours in 81 dogs and 14 cats showed a response rate higher than 70%; more than 50% of the pets were cured when DDW was used as a single treatment or in combination with surgery.