Authors: G. Somlyiai, G. Laskay, Gy. Jakli, etc
Synthesis and applications of isotopically labeled compounds, 1997

Abstract

It is known that deuterium/hydrogen (D/H) mass ratio is the largest of stable isotopes of the same element, causing differences in the physical and chemical behavior between the two isotopes.

The experiments carried out so far with D2O in different biological systems and other experiments which revealed the importance of hydrogen bond or intracellular pH in biological processes have neglected the naturally occurring deuterium, in spite of the fact that the concentration of D is about 150 ppm (over 16 mM) in surface water and more than 10 mM in living organisms.

The results revealed that due to D-depletion the non-tumorous L929 fibroblast cells required longer time to multiply in vitro and DDW caused tumor regression in mice. Our recent results suggest that NOD may have a central role either in cell cycle regulations or in apoptosis.